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目的：研究转染反义碱性成纤维细胞生长因子（ｂＦＧＦ）寡核苷酸（ＯＤＮ）对培养的自发性高血压大鼠（ＳＨＲ）主动脉平滑肌细胞（ＳＭＣ）生长的影响．方法：用脂质体介导法将反义ｂＦＧＦＯＤＮ转入ＳＭＣ内，用Ｎｏｒｔｈｅｒｎ杂交检测ｂＦＧＦ基因表达，并测定［３Ｈ］ｔｈｙｍｉｄｉｎｅ掺入和细胞计数．结果：转染反义ｂＦＧＦＯＤＮ（５μｍｏｌ·Ｌ－１）几乎完全抑制血管紧张素Ⅱ（ＡｎｇⅡ，１μｍｏｌ·Ｌ－１）诱导增高的ｂＦＧＦｍＲＮＡ表达和明显抑制ＳＭＣ增殖，在基础状态和ＡｎｇⅡ刺激条件下，［３Ｈ］ｔｈｙｍｉｄｉｎｅ掺入分别被抑制２６５％（Ｐ＜００１）和４２０％（Ｐ＜００１），细胞数分别被抑制１７３％（Ｐ＜００１）和２２２％（Ｐ＜００１）．结论：反义ｂＦＧＦＯＤＮ能有效抑制ＡｎｇⅡ诱导的ｂＦＧＦ基因表达和ＳＭＣ增殖．
Objective: To investigate the effects of transfection of antisense basic fibroblast growth factor (bFGF) ODN on the growth of aortic smooth muscle cells (SMCs) in spontaneously hypertensive rats (SHRs). Methods: Antisense bFGFODN was transfected into SMCs by liposome - mediated method. The expression of bFGF gene was detected by Northern hybridization and the incorporation of [3H] thymidine and cell count were determined. Results: Transfection of antisense bFGFODN (5μmol·L-1) almost completely inhibited angiotensin Ⅱ (AngⅡ, 1μmol·L-1) -induced increased bFGF mRNA expression and significantly inhibited the proliferation of SMC. Under basal and AngⅡ-stimulated conditions, The [3H] thymidine incorporation was inhibited by 265% (P <001) and 420% (P <001) respectively, and the cell number was inhibited by 173% (P <001) and 22 2% (P <0 01). Conclusion: Antisense bFGFODN can effectively inhibit Ang Ⅱ-induced bFGF gene expression and SMC proliferation.