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目的 探讨柚皮苷对H2O2诱导的H9c2心肌细胞凋亡的保护作用及机制.方法 体外培养H9c2心肌细胞,用H2O2诱导建立细胞凋亡模型.实验设对照组、模型组、柚皮苷低、中、高剂量组(10、20、40 μmol/L),噻唑蓝法检测细胞活力并用显微镜观察各组细胞形态;原位末端标记法检测H9c2心肌细胞凋亡情况;RT-PCR及Westemblot法检测凋亡相关因子Bcl-2、Bax、caspase-3 mRNA及蛋白表达.结果 与对照组比较,模型组细胞凋亡率[(17.2±2.1)％]明显升高(P＜0.01);与模型组比较,10、20、40 μmol/L柚皮苷组细胞凋亡率[分别为(10.7±1.9)％、(5.7±1.2)％、(6.4±1.5)％]均下降(均P＜0.05).与对照组比较,模型组H9c2心肌细胞Bcl-2蛋白表达水平(0.76±0.16)明显下调,Bax、caspase-3蛋白表达水平[分别为(5.42±0.52)、(1.09±0.11)]均上调(均P＜0.01);与模型组比较,10、20、40 μmol/L柚皮苷组H9c2心肌细胞Bcl-2蛋白表达水平[分别为(1.37±0.11)、(1.65±0.09)、(1.65±0.15)]均上调,Bax、caspase-3蛋白表达水平[分别为(2.78±0.55)、(3.43±0.15)、(2.69±0.26)和(0.59±0.08)、(0.77±0.06)、(0.82±0.05)]均下调(均P＜0.05).结论 柚皮苷对H2O2诱导的H9c2心肌细胞损伤具有一定保护作用,其机制可能与其对凋亡信号途径的抑制作用有关.“,”Objective To examine the protective effect and mechanism of naringin on H2O2-induced apoptosis of H9c2 myocardial cells.Methods H9c2 myocardial cells were cultured in vitro and a H9c2 myocardial cell apoptosis model was induced with hydrogen peroxide (H2O2).Five H9c2 myocardial cell groups were established:a control,model,low-,moderate-,and high-dose (10,20,and 40 μmol/L) naringin pretreatment group.Cell viability was detected with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and cell morphology was observed with microscope.The apoptosis of H9c2 myocardial cells was detected with terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) method.The expressions of B cell lymphoma 2 (Bcl-2),BCL2-associated X protein (Bax),caspase-3 mRNA and protein were detected with real-time reverse transcription PCR (RT-PCR) and Western blot.Results Compared with that of the control group,the apoptotic rate (17.2 ± 2.％) of the model group was significantly higher (P ＜ 0.01);compared with that the model group,the apoptotic rate of low-,moderate-,and high-dose naringin pretreatment group were significantly decreased (10.7 ± 1.9％,5.7 ± 1.2％,and 6.4 ± 1.5％) (all P ＜ 0.05).The Bcl-2 protein expression (0.76 ± 0.16)in H9c2 cardiomyocytes of the model group was significantly lower and the protein expressions of Bax and caspase-3 were significantly increased compared to those of the control group (all P ＜ 0.01).The protein expressions of Bcl-2 (1.37 ± 0.11,1.65 ± 0.09,andl.65 ± 0.15) in H9c2 myocardial cells of low-,moderate-,and high-dose naringin pretreatment group were significantly increased;while those of Bax (2.78 ± 0.55,3.43 ± 0.15,and 2.69 ± 0.26) and caspase-3 (0.59 ± 0.08,0.77 ± 0.06,and 0.82 ± 0.05) were significantly decreased compared to those of the control group (all P ＜ 0.05).Conclusion Naringin could inhibit apoptosis of H9c2 myocardial cells induced by H2O2,which may be related to its inhibitory effect on the apoptotic signaling pathway.