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本实验利用国内建立的小鼠可移植性瘤株子宮颈癌第27号(U_(27))癌细胞悬液脚掌皮下注射建立淋巴道转移实验模型,并对淋巴道转移过程、特点和淋巴结免疫反应的形态学变化进行了观察。实验结果发现,U_(27)癌细胞在局部经过24小时以上静止期后,便开始增殖并形成癌巢。癌细胞可经过淋巴管进入淋巴液中,4天后在局部淋巴结可查见癌细胞。癌细胞可在边缘窦定居繁殖形成转移灶,然后可经中间窦到达髓窦,再经输出淋巴管运行到远部淋巴结。在第14天远部淋巴结内查见癌细胞。在实验晚期部分小鼠肺内也发现转移癌灶形成。淋巴结早期反应为副皮质区明显扩大,免疫母细胞数显著增加。到实验晚期生发中心扩大融合,髓索内浆细胞显著增加。实验结果表明该模型具有以下特点:(1)取材国内自己建立的瘤株;(2)转移率高,而且重复性强;(3)到晚期可合并肺转移等。因此是一个较为理想的淋巴道转移实验模型。
In this experiment, an experimental model of lymphatic metastasis was established by subcutaneous injection of a mouse transplantable tumor cell line, cervical cancer No.27 (U_(27)), in the footpad, and the lymphatic metastasis process, characteristics, and lymph node immunity were studied. The morphological changes of the reaction were observed. The experimental results showed that U_(27) cancer cells began to proliferate and form cancer nests after a local resting period of more than 24 hours. Cancer cells can enter lymphatics via lymphatic vessels. Cancer cells can be found in local lymph nodes 4 days later. Cancer cells can colonize marginal sinuses to form metastases, which can then reach the medullary sinus through the middle sinuses and then travel to the distal lymph nodes via the output lymphatic vessels. On the 14th day, cancer cells were seen in the distal lymph nodes. Metastatic foci formation was also found in the lungs of some mice during the late stage of the experiment. The early response of the lymph nodes was marked expansion of the paracortical area and the number of immune mother cells was significantly increased. At the end of the experiment, the germinal center expanded and the plasma cells inside the cord increased significantly. The experimental results show that the model has the following characteristics: (1) The domestically established tumor strains are obtained; (2) the metastasis rate is high and the reproducibility is strong; (3) lung metastasis can be combined with the late stage. Therefore, it is an ideal experimental model of lymphatic metastasis.