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目的:探讨复方地黄对阿尔茨海默病(Alzheimer’s disease,AD)大鼠海马GDNF mRNA表达的影响。方法:通过Morris水迷宫筛选40只健康Wistar大鼠随机分出正常组10只(常规饮水和饲料,不给予任何处理),其余3组大鼠均采用链脲佐菌素(STZ)致AD模型,模型组10只造模后不进行治疗。造模后第6天开始治疗,安理申组(0.4 g.kg-1.d-1)和复方地黄组(含生药10 g.kg-1.d-1),每组10只,疗程4周。用Morris水迷宫检测大鼠的学习记忆能力,用原位杂交法和RT-PCR检测大鼠海马胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)GDNF mRNA的表达。结果:模型组大鼠学习能力比正常组降低(P<0.05),复方地黄大鼠上述变化明显改善,与模型组差异显著(P<0.05)。与正常组大鼠比较,模型组大鼠海马GDNF mRNA表达降低明显(P<0.05);与模型组比较,复方地黄组大鼠海马GDNF mRNA表达明显增加(P<0.05)。结论:复方地黄可能通过增强AD大鼠海马GDNF mRNA表达而起到对神经保护作用。
Objective: To investigate the effect of compound rehmannia root on the expression of GDNF mRNA in the hippocampus of Alzheimer’s disease (AD) rats. Methods: Forty healthy Wistar rats were screened by Morris water maze for 10 rats in the normal group (regular drinking water and feed without any treatment). The other 3 rats were treated with streptozotocin (STZ) induced AD model , Model group 10 models without treatment. The treatment was started on the 6th day after the model was established. Allixin (0.4 g.kg-1.d-1) and Compound Rehmanniae (10 g.kg-1.d-1 containing crude drug) week. The learning and memory abilities of rats were detected by Morris water maze and the expression of GDNF mRNA of hippocampus glial cell line-derived neurotrophic factor (GDNF) was detected by in situ hybridization and RT-PCR. Results: The learning ability of the model group was lower than that of the normal group (P <0.05). The changes of the compound Rehmannia glutinosa group were significantly improved (P <0.05). Compared with the normal group, the expression of GDNF mRNA in the hippocampus of the model group decreased significantly (P <0.05). Compared with the model group, the expression of GDNF mRNA in the hippocampus of the compound group was significantly increased (P <0.05). Conclusion: Compound Rehmannia may play a neuroprotective role by enhancing GDNF mRNA expression in the hippocampus of AD rats.