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目的探讨葡萄籽提取物F3(儿茶素高聚体,聚合度为10-33)对人恶性胶质瘤细胞株U87细胞增殖和趋化的影响。方法采用MTT法检测F3对U87细胞的增殖抑制作用。光镜及AO/EB染色观察细胞形态的变化。Boyden小室法探讨F3对U87的体外趋化作用。结果F3能抑制U87细胞的增殖,其作用呈明显的时间和剂量依赖性,给药24h、48h、72h的IC50分别为88μg·mL-1、53μg·mL-1、41μg·mL-1。光镜及AO/EB染色观察F3(10μg·mL-1、30μg·mL-1、60μg·mL-1、100μg·mL-1)作用24h使U87细胞形态发生明显改变。F3(1.0μg·mL-1、2.5μg·mL-1、5.0μg·mL-1、10.0μg·mL-1)预先作用1h能抑制FPR激动剂fMLF(10nM)诱导的U87细胞的趋化,显示一定的剂量依赖性关系。结论F3可抑制U87细胞的增殖,并可抑制FPR激动剂fMLF诱导的U87细胞的趋化。
Objective To investigate the effects of grape seed extract F3 (catechin polymer, degree of polymerization 10-33) on the proliferation and chemotaxis of human malignant glioma cell line U87. Methods The inhibitory effect of F3 on the proliferation of U87 cells was detected by MTT assay. Light microscopy and AO / EB staining observed cell morphology changes. Boyden chamber method to explore F3 on U87 chemotaxis in vitro. Results F3 could inhibit the proliferation of U87 cells in a time and dose-dependent manner. The IC50 of the cells were 88μg · mL-1, 53μg · mL-1, 41μg · mL-1 at 24h, 48h and 72h respectively. The morphological changes of U87 cells were observed by light microscope and AO / EB staining after treated with F3 (10μg · mL-1, 30μg · mL-1, 60μg · mL-1, 100μg · mL-1) Preincubation of F3 with 1.0μg · mL-1, 2.5μg · mL-1, 5.0μg · mL-1 and 10.0μg · mL-1 for 1 h inhibited chemotaxis of U87 cells induced by FPR agonist fMLF (10 nM) Show a certain dose-dependent relationship. Conclusions F3 inhibits the proliferation of U87 cells and inhibits the chemotaxis of U87 cells induced by FPR agonist FMLF.