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目的 探讨贵州省不同时间、不同地区伤寒流行菌株的遗传多态性及内在联系。方法 利用southern杂交技术 ,对贵州省 9个地区 2 6个县、市 195 9～ 1999年伤寒沙门菌分离株进行染色体DNA基因限制性酶切 16srDNA探针杂交图谱分析及药物敏感性试验。结果 分析发现选择的2 0 9株分属 2 6个RT型 ,以RT1和RT2为优势型 ;在局部发生伤寒流行时 ,均由独特RT型引起。耐药菌株以RT7和RT1型为主。结论 贵州省不同地区、不同时间的分离菌株在核糖体杂交图谱上有明显的多态性 ,分析认为具有多重耐药性以及存在众多克隆群菌株是引起贵州伤寒发病率居高不下的主要原因。
Objective To explore the genetic polymorphisms and internal relationships of epidemic strains of typhoid fever in Guizhou Province at different time and different places. Methods southern hybridization technique was used to analyze the 16S rDNA probe hybridization and drug sensitivity test of Salmonella typhi isolates from 269 counties in 9 counties and 9 counties in Guizhou Province from 1996 to 1999. The results showed that 2 0 9 RT strains were selected, which belonged to 2 RT genotypes, and RT1 and RT2 were the predominant ones. In the case of local typhoid epidemic, they were all caused by unique RT patterns. The resistant strains were mainly RT7 and RT1. Conclusion The isolates from different regions of Guizhou Province at different times have obvious polymorphism in the ribosomal hybridization map. It is considered that the multi-drug resistance and the presence of many clonal strains are the main causes of the high incidence of typhoid fever in Guizhou Province.