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以马齿苋子叶和下胚轴为外植体,研究了外源激素对其不定芽的诱导及不同浓度6-BA、NAA、GA3对不定芽伸长的影响,并测定了马齿苋子叶和下胚轴对草丁膦的敏感性。结果表明:MS+2.0mg/L 6-BA+100mg/L水解酪蛋白为下胚轴不定芽再生最适培养基,不定芽再生率达96.65%;MS+3.0mg/L 6-BA+100mg/L水解酪蛋白为子叶不定芽再生最适培养基,不定芽再生率达100%;最佳的不定芽继代培养的培养基为MS+0.5mg/L 6-BA+0.05mg/L NAA+0.2mg/L GA3;生根培养基为MS+2.0mg/L IBA,生根率达100%;以子叶和下胚轴为材料研究其对草丁膦的敏感性,发现子叶对草丁膦更敏感,0.050mg/L草丁膦对子叶的致死率达到89.0%;对下胚轴的致死率为71.5%。该研究建立了马齿苋植株高频再生体系,并确定了其对草丁膦的敏感性,可为该品种的基因工程研究提供必要的理论和实践基础。
In order to study the effects of exogenous hormones on adventitious buds and the effects of different concentrations of 6-BA, NAA and GA3 on the adventitious buds elongation, the leaf of horseshoe amaranth and hypocotyls were used as explants. And hypocotyl sensitivity to glufosinate. The results showed that MS + 2.0mg / L 6-BA + 100mg / L hydrolyzed casein was the optimum medium for adventitious bud regeneration of hypocotyls. The regeneration rate of adventitious buds reached 96.65%. MS + 3.0mg / L 6-BA + / L hydrolyzed casein is the best regeneration medium for adventitious buds regeneration, adventitious bud regeneration rate of 100%; best adventitious bud subculture medium MS + 0.5mg / L 6-BA + 0.05mg / L NAA + 0.2mg / L GA3. The rooting medium was MS + 2.0mg / L IBA with the rooting rate of 100%. The cotyledons and hypocotyls were used as materials to study their sensitivity to glufosinate. Sensitive, 0.050mg / L glufosinate cotyledon mortality reached 89.0%; on the hypocotyl mortality was 71.5%. The study established a high-frequency regeneration system of purslane plants and determined its sensitivity to glufosinate, which may provide the necessary theoretical and practical basis for genetic engineering of this variety.