论文部分内容阅读
目的 研究人类白细胞抗原 (human leukocyte antigen,HL A) DRB1等位基因与儿童特发性血小板减少性紫癜 (idiopathic thrombocytopenic purpura,ITP)的关系。方法 用聚合酶链反应 -序列特异的寡核苷酸探针杂交技术对 4 2例 ITP患儿进行了 HL A- DRB1等位基因分型 ,同时用改良的血小板抗原单抗特异性固相化法检测了其中 36例 ITP患儿血清中的抗 GP b/ a和抗 GPIb/ x自身抗体。结果 与健康对照相比 ,ITP患儿 HL A- DRB1* 17基因频率显著升高 (P<0 .0 5 ,RR=2 .76 ,EF=0 .10 6 4 ) ,而HL A- DRB1* 12 0 2基因频率显著降低 (P<0 .0 2 5 ,RR=0 .2 0 ,PF=0 .76 16 ) ;慢性难治性 ITP患儿与非难治性患儿相比 ,HL A- DRB1* 11基因频率显著升高 (χ2 =6 .0 91,P<0 .0 2 5 ) ,且具有 DRB1* 11的患儿主要(5 / 6 )为女性年长患儿 ;抗 GP b/ a及抗 GPIb/ x自身抗体的阳性率都与 HL A- DRB1* 0 2 (15 / 16 )基因显著相关 (P分别为 0 .0 2和 0 .0 1) ,但难治性和非难治性 ITP患儿间抗体阳性率差异无显著性 (P>0 .1)。结论 DRB1* 17可能与儿童 ITP的易感性有关 ,而 DRB1* 12 0 2则可能对儿童 ITP的发病具有保护作用 ;具有 DRB1* 11基因的患儿易发展为慢性难治性 ITP;血小板自身抗体与抗原表位的反应可能受DRB1*
Objective To study the relationship between HLA-DRB1 alleles and idiopathic thrombocytopenic purpura (ITP) in children. Methods A total of 42 cases of ITP children were genotyped with HLA-DRB1 alleles by polymerase chain reaction-sequence-specific oligonucleotide probe hybridization and modified with a modified monoclonal antibody specific for platelet antigen Methods Anti-GP b / a and anti-GPIb / x autoantibodies were detected in serum of 36 children with ITP. Results The frequency of HLA-DRB1 * 17 gene in ITP children was significantly higher than that in healthy controls (P <0.05, RR = 2.76, EF = 0.1064) 12 0 2 gene frequency was significantly lower (P0.05, RR = 0.20, PF = 0.76 16); chronic refractory ITP children compared with non-refractory children, HL A- The frequency of DRB1 * 11 gene was significantly increased (χ2 = 6 .091, P <0.05), and the majority of children with DRB1 * 11 were female older children. The anti-GPb / a and anti-GPIb / x autoantibodies were significantly associated with HL-DRB1 * 0 2 (15/16) genes (P = 0.002 and 0.01, respectively), but refractory and non-refractory There was no significant difference in antibody positive rate among children with ITP (P> 0. 1). Conclusion DRB1 * 17 may be related to the susceptibility of children with ITP, while DRB1 * 12 0 2 may have a protective effect on the development of childhood ITP. Children with DRB1 * 11 may develop chronic refractory ITP. Platelet autoantibodies Reactions with epitopes may be affected by DRB1 *