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目的:建立以HPLC法测定三七血伤宁胶囊中人参皂苷Rg_1含量的方法。方法:采用Thermo C_(18)色谱柱(200mm×4.6 mm,5μm),以乙腈:0.4%磷酸=20:80为流动相,检测波长:203 nm,流速1.0 ml·min~(-1)。结果:人参皂苷Rg_1在0.441 6～2.208 0μg范围内线性关系良好,r=0.999 9,平均加样回收率为99.5%。结论:本方法操作简便、方法可靠,结果准确、灵敏度高,重复性好,可作为该产品质量控制的方法。
Objective: To establish a method for the determination of ginsenoside Rg 1 in Sanqi Xueyouning capsule by HPLC. Methods: The mobile phase was acetonitrile (0.4% phosphoric acid) = 20:80 on a Thermo C 18 column (200 mm × 4.6 mm, 5 μm). The detection wavelength was 203 nm and the flow rate was 1.0 ml · min -1. Results: Ginsenoside Rg_1 showed good linearity in the range of 0.441 6 ~ 2.208 0 μg with r = 0.999 9 and the average recovery was 99.5%. Conclusion: The method is simple and convenient, the method is reliable, the result is accurate, the sensitivity is high and the repeatability is good. It can be used as the method of product quality control.