论文部分内容阅读
目的探讨晚期糖基化终产物受体(RAGE)2184A/G基因的多态性与肺癌发生发展的关系。方法选取年龄、性别匹配的275例肺癌患者和126名健康者,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测RAGE 2184A/G基因多态性的分布频率。结果 2148A/G多态性的分析中,健康者中仅发现野生型A等位基因,基因频率为100.0%。3种不同类型肺癌均显示最小等位基因G条带,其中腺癌患者G等位基因频率为7.0%,鳞癌为6.1%,小细胞癌为7.7%(均P<0.05),未能检测出纯合的GG。在不同分期的肺癌患者中,腺癌患者AG基因型频率为14.0%,早中期患者AG基因型频率为3.2%,晚期肺癌AG基因型频率明显升高,为20.4%(P=0.048)。鳞癌患者AG基因型频率为12.2%,早中期鳞癌患者AG基因型频率为8.0%,而晚期鳞癌患者AG基因型频率可高达16.7%(P=0.05)。小细胞癌患者AG基因型频率为15.4%。在腺癌和鳞癌患者中,男性AA基因型频率明显低于女性,分别为81.4%和90.7%以及81.3%和100.0%;而在小细胞癌患者中,男性AA基因型频率(100.0%)明显高于女性患者(50.0%)。结论 RAGE 2148A/G基因多态性与肺癌的发生密切相关。在肺癌和腺癌中,AG基因型频率与肺癌的恶性程度呈正相关,同时肺癌患者2184A/G多态性具有显著的性别差异。RAGE 2148A/G基因有望成为肺癌基因治疗的靶向之一。
Objective To investigate the relationship between the polymorphism of advanced glycation end product receptor (RAGE) 2184A / G gene and the occurrence and development of lung cancer. Methods A total of 275 patients with lung cancer and 126 healthy controls matched for age and gender were enrolled. The frequency of RAGE 2184A / G polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results In the analysis of 2148A / G polymorphism, only the wild type A allele was found in healthy persons, the gene frequency was 100.0%. The three alleles of G alleles were all detected in 3 different types of lung cancer. The frequency of G allele was 7.0% in adenocarcinoma, 6.1% in squamous cell carcinoma and 7.7% in small cell carcinoma (all P <0.05) Out of pure GG. Among patients with different stages of lung cancer, AG genotype frequency was 14.0% in adenocarcinoma patients, AG genotype frequency was 3.2% in early-middle-term patients, and AG genotype frequency was significantly higher in advanced lung cancer patients (20.4%, P = 0.048). The frequency of AG genotype in patients with squamous cell carcinoma was 12.2%. The frequency of AG genotype in patients with squamous cell carcinoma was 8.0% in early-stage squamous cell carcinoma and 16.7% (P = 0.05) in patients with advanced squamous cell carcinoma. AG genotype in patients with small cell carcinoma was 15.4%. In patients with adenocarcinoma and squamous cell carcinoma, the frequency of AA genotype was significantly lower in males than in females (81.4% vs. 90.7%, 81.3%, 100.0%, respectively); in patients with small cell carcinoma, the frequency of AA genotypes was 100.0% Significantly higher than female patients (50.0%). Conclusion RAGE 2148A / G polymorphism is closely related to the occurrence of lung cancer. In lung cancer and adenocarcinoma, the frequency of AG genotype was positively correlated with the degree of malignancy of lung cancer, meanwhile there was a significant gender difference in 2184A / G polymorphism in patients with lung cancer. RAGE 2148A / G gene is expected to become one of the targets of lung cancer gene therapy.