论文部分内容阅读
基于腺酶催化尿素分解产生氨，以氨气敏电极为基础电极，用含脲酶丰富的谷氨酸棒状杆菌研制成测定尿素的微生物传感器。在３０℃、ｐＨ８．０、０．１ｍｏｌ／Ｌ磷酸盐缓冲液中，该传感器的线性范围为１．１×１０￣（－４）～１．４×１０￣（－２）ｍｏｌ／Ｌ，斜率为５１．２ｍＶ／ｄｅｃａｄｅ，检测下限为１．０×１０￣（－５）ｍｏｌ／Ｌ，寿命可达４５ｄ．考察了传感器响应初速和底物浓度之间的关系，测定了微生物膜中脲酶的表观米氏常数Ｋ＿ｍ及最大响应初速ｖ＿ｍ．
Based on the ammonia-catalyzed decomposition of urea to produce ammonia, ammonia-based electrode as the base electrode, urease-rich Corynebacterium glutamicum was developed into the determination of urea microbial sensor. The linear range of this sensor was 1.1 × 10 -4 to 1.4 × 10 -2 mol / L in pH 8.0 and 0.1 mol / L phosphate buffer solution at 30 ℃. The slope is 51.2mV / decade, the detection limit is 1.0 × 10 ~ (-5) mol / L and the life span is up to 45 days. The relationship between the initial velocity of the sensor and substrate concentration was investigated. The apparent Michaelis constant K_m and the maximum response velocity v_m of urease in the microbial membrane were measured.