论文部分内容阅读
目的了解广州市艾滋病病毒Ⅰ型(HIV-1)流行株的亚型及分布情况。方法从2004-2005年采集的92份HIV-1抗体阳性者抗凝全血中,提取前病毒DNA,经套式聚合酶链反应(Nested-PCR)扩增gag和env区基因并测定序列,与国际标准参考序列比较确定亚型。结果经系统进化树分析,92份样本中34份为CRF01-AE,40份为CRF07-BC,7份为CRF08-BC,3份为欧美B,6份为泰国B(B’),2份为C。结论广州市HIV-1流行株以CRF01-AE和CRF07-BC为主,也存在CRF08-BC、B、B’及C亚型。
Objective To understand the subtypes and distribution of HIV-1 strains in Guangzhou. Methods DNA was extracted from 92 HIV-1 positive anticoagulant whole blood samples collected from 2004 to 2005. Genomic DNA of gag and env were amplified by Nested-PCR and sequenced. Subtype comparison with international standard reference sequences. Results According to phylogenetic tree analysis, 34 out of 92 samples were CRF01-AE, 40 were CRF07-BC, 7 were CRF08-BC, 3 were European and American B, 6 were Thai B (B ’), 2 C Conclusion CRF01-AE and CRF07-BC are the predominant HIV-1 strains in Guangzhou, and CRF08-BC, B, B ’and C subtypes also exist.