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目的 :探讨CyclinD1基因的表达与喉癌发生发展的关系。方法 :应用S -P免疫组化方法检测10例声带息肉组织及44例喉鳞癌组织中CyclinD1蛋白的表达。结果 :CyclinD1在喉癌组织中的阳性表达率为68.3%(30/44) ,在声带息肉组织中的阳性率为30.0%(3/10) ,二者差别有统计学意义 ,P<0.05,而在癌旁正常组织中未见表达。CyclinD1蛋白表达与喉癌患者年龄、淋巴结转移无明显相关 ,而与组织分化程度及临床分期呈正相关 ,低分化喉癌的阳性表达率明显比高分化、中分化喉癌高 ,P<0.05;Ⅲ、Ⅳ期喉癌的阳性表达率明显高于Ⅰ -Ⅱ期 ,P<0.05.结论 :CyclinD1过度表达是喉癌发生发展中的重要事件 ,并可作为判断喉癌恶性程度及预后的指标
Objective: To investigate the relationship between the expression of CyclinD1 gene and the development of laryngeal carcinoma. Methods: The expression of CyclinD1 protein in 10 cases of vocal cord polyp and 44 cases of laryngeal squamous cell carcinoma was detected by S-P immunohistochemistry. Results: The positive expression rate of CyclinD1 in laryngeal carcinoma was 68.3% (30/44), and the positive rate in vocal cord polyps was 30.0% (3/10), the difference was statistically significant (P <0.05) In normal tissue adjacent to no expression. The expression of CyclinD1 in laryngeal cancer patients with age and lymph node metastasis was not significantly correlated with the degree of differentiation and clinical stage was positively correlated, poorly differentiated laryngeal cancer was significantly higher than the well-differentiated, moderately differentiated laryngeal cancer, P <0.05; Ⅲ , The positive expression rate of stage Ⅳ laryngeal carcinoma was significantly higher than that of stage Ⅰ -Ⅱ, P <0.05.Conclusion: Overexpression of CyclinD1 is an important event in the development and progression of laryngeal carcinoma and can be used as an indicator of malignancy and prognosis of laryngeal carcinoma