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背景:国际上常用的肾移植慢性排斥模型是Fisher→Lewis大鼠肾移植模型等,但这些模型在国内均较难以获得并且价格昂贵,大规模使用受到了一定的限制。目的:探索建立SD→Wistar大鼠肾移植慢性排斥模型的新方法。方法:将56对SD→Wistar大鼠作左肾原位移植,受体自身右肾保留作为内对照。23只成功移植的受体大鼠随机分为模型组(n=15)和对照组(n=8)。模型组大鼠在移植后给予10 d的小剂量环孢素微乳化剂[2 mg/(kg·d),腹腔注射],对照组大鼠未给予免疫抑制治疗。结果与结论:对照组大鼠所有移植肾均在4周内出现不可逆的急性排斥反应,移植肾坏死;模型组大鼠移植后4,8,12周时均可见移植肾中出现中等度的炎症细胞浸润,在移植后12周内出现典型的慢性排斥组织病理学改变,其Banff总分随移植后时间的延长而升高。2组大鼠所有受体的自身右肾均没有出现组织病理学改变。模型组大鼠移植后第4天环孢素浓度谷值为(153.2±17.1)μg/L。说明通过给予肾移植的SD→Wistar大鼠受体10 d小剂量的环孢素微乳化制剂(2 mg/kg),可使移植肾出现中等度的炎症细胞浸润并于12周内形成慢性排斥的典型组织病理学改变,可作为研究肾移植慢性排斥的动物模型。
BACKGROUND: The chronic rejection model of kidney transplantation commonly used in the world is the kidney transplant model of Fisher → Lewis rats. However, these models are hard to be obtained in China and are expensive. The large-scale use is limited. Objective: To explore a new method of establishing a chronic rejection model of renal transplantation in SD → Wistar rats. Methods: 56 SD → Wistar rats were transplanted into the left kidney in situ and the recipient’s own right kidney was reserved as an internal control. Twenty-three successfully transplanted recipients were randomly divided into a model group (n = 15) and a control group (n = 8). The model rats were given a small dose of cyclosporine microemulsifier [2 mg / (kg · d), intraperitoneal injection] for 10 days after transplantation. The rats in the control group were not given immunosuppressive therapy. RESULTS AND CONCLUSION: All the allograft kidneys in control group showed irreversible acute rejection and renal allograft necrosis within 4 weeks. At 4, 8 and 12 weeks after transplantation, moderate inflammatory cell infiltration , Typical chronic rejection histopathological changes occurred within 12 weeks after transplantation. The total score of Banff increased with time after transplantation. There was no histopathological changes in the right kidney of all recipients in both groups. At the 4th day after transplantation, the trough concentration of cyclosporine in the model group was (153.2 ± 17.1) μg / L. It shows that moderate renal infiltration of renal grafts can be induced by a small dose of cyclosporine micro-emulsifying agent (2 mg / kg) for 10 days in SD → Wistar rat recipients after renal transplantation and chronic rejection in 12 weeks The typical histopathological changes, can be used as animal models of chronic rejection of renal transplantation.