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以含有华南4种稻瘿蚊生物型显性抗性基因Gm6的籼稻材料KG18为供体,三系优质恢复系广恢998为受体,通过杂交、多次回交和自交结合分子标记辅助选择技术,将Gm6基因导入广恢998中。综合目标基因的分子检测、田间农艺性状表现以及遗传背景的回复率,筛选获得6个株系。采用稻瘿蚊生物型1和生物型4对这些改良株系进行人工接虫鉴定,6个改良株系的标葱率为0,表现免疫,而对照标葱率均在98%以上,表现高感;由改良株系组配的杂种F1标葱率在10%～20%,表现中抗。农艺性状分析表明,除1个株系在千粒重方面与对照广恢998有显著差异外,其余改良株系性状与对照无显著差异。
In this study, KG18, a indica rice germplasm with dominant Dm gene Gm6 in South China, was used as donor. Three lines of high quality restorer Guanghui 998 were used as recipients. Through hybridization, multiple backcrossing and selfing, molecular marker assisted selection Technology, the Gm6 gene into Guang Hui 998. Based on the molecular detection of the target gene, the performance of agronomic traits in the field and the response rate of the genetic background, six lines were screened. These improved strains were identified by artificial insects using the biotype 1 and biotype 4 of rice gall midge mosquito, the standard onion rate of 6 improved lines was 0, showing immunization, while the control rate of onion were above 98%, high performance Sense; by the improved line hybrid F1 hybrid onion rate of 10% to 20%, the performance of resistance. Agronomic traits analysis showed that there were no significant differences in the traits of the other improved lines except the one line with the control Guanghui 998 in terms of 1000-grain weight.