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目的:探讨碘化N-正丁基氟哌啶醇(F2)对兔颈总动脉球囊损伤后内膜及平滑肌细胞增殖的影响。方法:30只新西兰兔随机分为假手术组、模型组、F2高剂量给药组(2mg/kg)、F2中剂量给药组(1mg/kg)、F2低剂量给药组(0.5mg/kg)。模型组及F2各给药组行左侧颈总动脉球囊损伤,各组分别于术后7d取颈总动脉段,常规病理切片,HE染色,免疫组化法测定α-actin、增殖细胞核抗原(PCNA)的表达水平。结果:与假手术组比较,模型组术后7d血管内膜厚度、内膜面积、内膜厚度/中膜厚度、内膜面积/中膜面积、血管壁细胞PCNA表达显著增加(P<0.01),F2剂量依赖地降低上述指标。与假手术组比较,模型组血管壁α-actin阳性染色减少,F2各给药组可增加血管壁平滑肌细胞中α-actin阳性染色率。结论:F2能抑制兔颈总动脉机械损伤后血管内膜和平滑肌细胞的增殖。
Objective: To investigate the effect of n-butyl haloperidol iodide (F2) on the proliferation of intima and smooth muscle cells after balloon injury in the common carotid arteries of rabbits. Methods: Thirty New Zealand rabbits were randomly divided into sham operation group, model group, F2 high dose group (2mg / kg), F2 medium dose group (1mg / kg), F2 low dose group kg). The left common carotid artery balloon injury was induced in the model group and the F2 administration group. The common carotid arteries were taken from the rats in each group on the 7th day after operation. The pathological sections were stained with HE. The expression of α-actin, proliferating cell nuclear antigen (PCNA) expression levels. Results: Compared with the sham operation group, the intima thickness, intima thickness, intima thickness / intima thickness, intima area / intima area, and PCNA expression in the model group were significantly increased at 7th day after operation (P <0.01) , F2 dose-dependently reduce the above indicators. Compared with the sham-operation group, α-actin staining in the model group decreased, while F2 administration group increased the positive staining rate of α-actin in the vascular smooth muscle cells. Conclusion: F2 can inhibit the proliferation of vascular intima and smooth muscle cells after mechanical injury of rabbit common carotid arteries.