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干旱是影响玉米生产的重要限制因素 ,特别是花期对干旱胁迫非常敏感。本研究通过对玉米L0 5 0×B73的 180个F2 :3 家系进行开花期干旱胁迫处理和分子标记鉴定 ,重点对开花相关性状进行了数量性状位点 (QTL)分析。结果表明 ,在干旱胁迫处理条件下 ,存在与出苗到抽雄天数有关的 6个QTL ,位于第 1、6、9染色体上各 1个 ,位于第 3染色体上有 3个 ,共可解释的表现型变异为 5 5 .0 % ;基于出苗到散粉天数检测到 4个QTL ,其中两个位于第 3染色体上 ,位于第 1、2染色体上各 1个 ,共可解释的表型变异为 5 2 .8% ;对出苗到吐丝天数检测到分别位于第 3、6染色体上的 2个QTL ,共可解释的表现型变异为 2 0 .4 % ;对抽雄至吐丝间隔天数 (ASI)只检测位于第 6染色体上的 1个QTL ,可解释 6 .5 %的表现型变异。而正常灌溉环境下 ,检测到出苗到抽雄天数检测到 1个QTL ,位于第 9染色体上 ,可解释的变异为 15 .0 % ;对出苗到散粉天数检测到 3个QTL ,位于第 1、3、9染色体上 ,共可解释的变异为 5 5 .0 % ;对出苗到吐丝天数检测到 4个QTL ,分别位于第 1、2、3、7染色体上。共可解释表现型变异的 4 6 .8% ;对ASI检测到分别位于第 2、6染色体上的 2个QTL ,可解释的变异为 15 .5 %。这些QTL的基因效应以显性与超显性为主
Drought is an important limiting factor on corn production, especially when flowering is very sensitive to drought stress. In this study, 180 F 2: 3 pedigrees of maize L0 5 0 × B73 were subjected to flowering drought stress and molecular marker identification. Quantitative trait loci (QTL) analysis of flowering-related traits was carried out. The results showed that there were 6 QTLs related to the number of days from emergence to anthesis under drought stress treatment, one on chromosome 1, 6 and 9, and 3 on chromosome 3, which could explain the phenotype The variation was 5 5 .0%. Four QTLs were detected based on days of emergence to scattered powder. Two of them were located on chromosome 3 and one on chromosome 1 and 2, respectively. The total phenotypic variation was 52. 8%. The two QTLs detected on the 3rd and 6th chromosomes respectively from the days of emergence to the number of silking days were 20.4% of the total phenotypic variation. The ASI interval from tasseling to silking was only detected One QTL on chromosome 6 explained 6.5% of the phenotypic variation. Under normal irrigation conditions, one QTL was detected from the days of emergence to the day of tasselling, which was explained by 15.0% on chromosome 9. Three QTLs were detected in days of emergence to loose powder, On chromosome 9, a total of 5 5 .0% of the variance was explained. Four QTLs were detected on the days of emergence to silking, which were located on chromosomes 1, 2 and 3, respectively. A total of 46.8% of the phenotypic variation could be explained. For the two QTLs detected by ASI on chromosomes 2 and 6, the explained variance was 15.5%. The genetic effects of these QTLs were dominant and super dominant