论文部分内容阅读
目的:研究枯草溶菌素转换酶9(proproteinconvertase subtilisin/kexin type 9,PCSK9)在大鼠心肌缺血再灌注损伤中的表达变化。方法:采用大鼠心肌缺血再灌注损伤模型,大鼠分4组:对照组(n=5)、实验组分别为缺血45 min再灌注6 h、12 h及24 h组(各组n=5)。实验终点时,收集血液及缺血区心肌组织,全自动生化分析仪测定心肌酶谱(CK、CK-MB和LDH),HE染色观察心肌组织病理学改变,分别采用实时定量PCR及Western blot方法检测心肌PCSK9 mRNA及蛋白水平表达的改变。结果:与对照组相比,实验组CK、CK-MB和LDH在6 h和12 h组均明显升高有统计学意义,24 h组恢复正常;HE染色发现心肌组织病理损伤随着再灌注时间延长而加重;实验各组心肌PCSK9 mRNA及蛋白水平表达均显著高于对照组,并且随着再灌注时间的延长表达水平逐渐增高。结论:PCSK9可能参与心肌缺血再灌注损伤的病理生理过程。
Objective: To study the expression changes of subtilisin / kexin type 9 (PCSK9) in myocardial ischemia-reperfusion injury in rats. Methods: The rat model of myocardial ischemia-reperfusion injury was divided into 4 groups: control group (n = 5), experimental group were reperfusion at 45 min after reperfusion for 6 h, 12 h and 24 h groups = 5). At the end of the experiment, the myocardial tissue of blood and ischemic area were collected. The myocardial enzymes (CK, CK-MB and LDH) were measured by automatic biochemical analyzer. The pathological changes of myocardial tissue were observed by HE staining. Real-time PCR and Western blot The changes of myocardial PCSK9 mRNA and protein expression were detected. Results: Compared with the control group, CK, CK-MB and LDH in the experimental group increased significantly at 6 h and 12 h, and returned to normal at 24 h. The pathological changes of myocardium with HE staining showed that with the reperfusion Prolonged and aggravate. The expression of PCSK9 mRNA and protein in each group were significantly higher than those in control group, and the expression level of PCSK9 gradually increased with the reperfusion time prolonging. Conclusion: PCSK9 may be involved in the pathophysiological process of myocardial ischemia-reperfusion injury.