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目的初步研究肽聚糖通过Toll样受体2(TLR2)诱导人早孕绒毛外滋养细胞株TEV-1细胞的凋亡及其意义。方法免疫细胞化学检测TLR2蛋白在TEV-1细胞的定位表达,不同浓度肽聚糖刺激TEV-1细胞后AnnexinV/PI流式细胞术检测细胞的凋亡率。结果①TEV-1细胞表达TLR2,且定位于细胞膜和细胞浆。②30μg/ml肽聚糖刺激TEV-1细胞24小时后与对照组比较,细胞凋亡率无明显增加(8.17±0.1%,P>0.05),而刺激时间延长至48h可诱导出明显增加的细胞凋亡(16.03±1.51%,P<0.01);大剂量组80μg/ml肽聚糖刺激TEV-1细胞24h、48h均可诱导明显增加的细胞凋亡(14.13±1.06%和51.67±1.56%,P<0.01)。结论人绒毛外滋养细胞株TEV-1表达TLR2,肽聚糖可通过TLR2诱导TEV-1细胞凋亡,且存在时间和剂量依赖性,提示宫内感染时G+菌可能通过TLR2介导胎盘滋养细胞凋亡来影响妊娠的结局。
Objective To study the effect of peptidoglycan on the apoptosis of human trophoblast cell line TEV-1 in early pregnancy induced by Toll-like receptor 2 (TLR2). Methods The localization of TLR2 protein in TEV-1 cells was detected by immunocytochemistry. The apoptosis rate of TEV-1 cells was detected by Annexin V / PI flow cytometry after the cells were incubated with different concentrations of peptidoglycan. Results ① The expression of TLR2 in TEV-1 cells localized in the plasma membrane and cytoplasm. ② After stimulated with 30μg / ml peptidoglycan for 24 hours, the apoptotic rate of TEV-1 cells was not significantly increased (8.17 ± 0.1%, P> 0.05) compared with the control group, while the stimulation time prolonged to 48h induced significantly increased cells (16.03 ± 1.51%, P <0.01). TEV-1 cells were stimulated by 80μg / ml peptidoglycan in high dose for 24 h and 48 h, respectively, which could induce significantly increased apoptosis (14.13 ± 1.06% and 51.67 ± 1.56% P <0.01). Conclusion Human chorionic villi trophoblast cell line TEV-1 expresses TLR2, and peptidoglycan can induce apoptosis of TEV-1 cells through TLR2 in a dose-and time-dependent manner, suggesting that G + bacteria may mediate placental trophoblast cells through TLR2 during intrauterine infection Apoptosis affects the outcome of pregnancy.