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本实验利用双抗体夹心ＥＬＩＳＡ、流式细胞测量术及淋巴细胞增殖试验观察了可溶性ＩＬ－２受体（ｓＩＬ－２Ｒ）释放与细胞膜ＩＬ－２受体（ｍＩＬ－２Ｒ）表达及淋巴细胞增殖反应的相关性。结果显示：①在不更换培养液的情况下，ＰＨＡ刺激的淋巴细胞培养上清中的ｓＩＬ－２Ｒ释放与ｍＩＬ－２Ｒ表达在７２ｈ内呈正相关（ｒ＝０．９４．Ｐ＜０．０５）；之后，ｍＩＬ－２Ｒ的表达逐渐减少，而ｓＩＬ－２Ｒ水平则继续缓慢增加。呈负相关（ｒ＝－０．９６．ｐ＜０．０５）；②在不同细胞浓度及不同ＰＨＡ浓度时，ｓＩＬ－２Ｒ释放与淋巴细胞增殖反应呈正相关（ｒ＝０．９６．ｐ＜０．０５；ｒ＝０９９．ｐ＜０．０１）；③１４位恶性肿瘤患者的ＰＨＡ刺激末梢血淋巴细胞培养上清中ｓＩＬ－２Ｒ水平与淋巴细胞增殖反应呈正相关（ｒ＝０．８６．ｐ＜０．０１）．上述结果提示：在培养７２ｈ内，ｓＩＬ－２Ｒ水平可反映ｍＩＬ－２Ｒ的表达状态；通过测定培养上清中ｓＩＬ－２Ｒ水平可间接了解淋巴细胞增殖情况。
In this study, the release of soluble IL-2 receptor (sIL-2R) and the expression of IL-2 receptor (mIL-2R) and lymphocyte proliferation were observed by double antibody sandwich ELISA, flow cytometry and lymphocyte proliferation assay Relevance. The results showed that: (1) The release of sIL-2R in the supernatant of PHA-stimulated lymphocytes was positively correlated with the expression of mIL-2R within 72 hours (r = 0.94, P <0.05) ; After that, the expression of mIL-2R gradually decreased while the level of sIL-2R continued to increase slowly. (R = -0.96.p <0.05). ②The release of sIL-2R was positively correlated with the proliferation of lymphocytes at different cell concentrations and different PHA concentrations (r = 0.96, p <0 .05; r = 099.p <0.01). ③ The level of sIL-2R in the supernatant of peripheral blood lymphocytes stimulated by PHA in 14 malignant tumors was positively correlated with lymphocyte proliferation (r = 0.86, p < 0.01). The above results suggest that the level of sIL-2R can reflect the expression of mIL-2R within 72 hours after culture, and the proliferation of lymphocytes can be indirectly obtained by measuring the level of sIL-2R in the culture supernatant.