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目的:阐述生物技术在雷公藤Tripterygium wilfordii hook减毒增效研究中的应用概况。方法:总结国内外相关文献,从生物靶向给药、酶和细胞对单体进行修饰、内生菌中分离有效成分以及整体发酵等方面归纳生物技术在雷公藤减毒增效研究中的应用。结果:通过将药物靶向运输到病变部位、对单体有效成分进行结构修饰、整体转化雷公藤以及从内生菌中分离有效成分等手段,生物技术能在单体有效成分和雷公藤整体水平上对雷公藤进行减毒增效。结论:采用模式菌和重组基因工程菌转化雷公藤各种减毒增效成分,同时积极筛选生物脱毒相关基因或毒理作用的最佳靶点并合成其抑制剂得到减毒成分将是雷公藤减毒增效研究的一个重要方向。
Objective: To summarize the application of biotechnology in Tripterygium Tripterygium wilfordii hook attenuated and synergistic research. Methods: To summarize relevant literatures at home and abroad, and to summarize the application of biotechnology in the study of attenuated and synergistic effects of Tripterygium wilfordii from the aspects of bio-targeted drug delivery, modification of monomers by enzymes and cells, isolation of effective components in endophyte, and overall fermentation . Results: By means of targeted drug delivery to diseased sites, structural modification of the effective components of the monomers, overall transformation of Tripterygium wilfordii, and isolation of effective components from endophytes, biotechnology can be applied to the active ingredients of the monomer and the overall level of Tripterygium On the triptolide to reduce toxicity and efficiency. CONCLUSION: It is a good idea to use the model bacteria and recombinant genetic engineering bacteria to transform various attenuated and synergistic components of Tripterygium wilfordii. At the same time, to actively screen for the best targets of biological detoxification related genes or toxicological effects and synthesize their inhibitors to obtain attenuated components, An important direction for the study of villus attenuating and efficiency improvement.