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目的:探讨慢性间歇低氧对大鼠认知功能及大脑环磷酸腺苷反应单元结合蛋白(CREB)表达的影响。方法:通过常压低氧仓建立间歇低氧大鼠模型,即对成年大鼠每90s交替给予10%氧和21%氧,每天8h,连续处理14d后,以Morris水迷宫观察大鼠空间学习记忆的变化,并采用RT-PCR法观察大鼠皮层区及海马区环磷酸腺苷反应单元结合蛋白mRNA的表达。结果:与常压对照组比较,常压间歇低氧组大鼠在Morris水迷宫中的空间学习记忆能力明显下降,表现为平均逃避潜伏期延长和游泳总距离增加。同时,大鼠皮层区及海马区CREBmR-NA的表达水平明显降低。结论:慢性间歇低氧可引起大鼠空间学习记忆能力下降,而大鼠这种慢性脑功能损害可能与大脑CREBmRNA的表达下调有关。
Objective: To investigate the effect of chronic intermittent hypoxia on cognitive function and expression of cyclic adenosine monophosphate (CREB) in rat brain. Methods: An intermittent hypoxic rat model was established by normal pressure hypoxia chamber. The adult rats were alternately treated with 10% oxygen and 21% oxygen every 90s for 8 hours. After continuous treatment for 14 days, Morris water maze was used to observe the spatial learning The changes of cAMP and cAMP mRNA expression in cortex and hippocampus were observed by RT-PCR. Results: Compared with the normal control group, the spatial learning and memory abilities of the rats in the intermittent hypoxia group were significantly decreased in the Morris water maze, showing the prolongation of the average escape latency and the increase of the total swimming distance. Meanwhile, the expression of CREBmR-NA in the cortex and hippocampus of rats decreased significantly. CONCLUSION: Chronic intermittent hypoxia can cause spatial learning and memory in rats decreased, while chronic brain damage in rats may be related to the down-regulation of CREB mRNA in the brain.