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对番茄种子芽期过氧化物酶（POD）同工酶与酯酶（EST）同工酶聚丙烯酰胺凝胶电泳（PAGE）方法进行了初步研究。结果表明，POD同工酶电泳酶样提取液以pH6．5～7.5的0.1．mol/L磷酸缓冲液十0．1％巯基乙醇较适宜；EST同工酶酶样提取液以pH6．0～70的0．1mol／LTris-柠檬酸＋0．1％巯基乙醇或0．1mol／L磷酸缓冲液＋0．1％巯基乙醇较适宜。常用的7.5％分离胶浓度不适宜番茄种子芽期POD和EST同工酶电泳分析，采用11．5％的分离胶能达到理想的分离效果。
POD isozymes and EST isoenzyme polyacrylamide gel electrophoresis (PAGE) were preliminarily studied in the seed germination of tomato. The results showed that POD isozyme electrophoresis enzyme-like extract 0.1 to pH 6.5 to 7.5. mol / L phosphate buffer ten 0.1% mercaptoethanol is more appropriate; EST isozyme enzyme-like extract pH6.0 ~ 70 0.1mol / LTris-citric acid + 0.1% mercaptoethanol or 0.1mol / L phosphate buffer + 0.1% mercaptoethanol is more suitable. The commonly used concentration of 7.5% separation gel is not suitable for tomato POD and EST isozyme electrophoresis analysis, using 11.5% separation gel can achieve the desired separation effect.