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茉莉酸是调控橡胶树创伤反应及产排胶的重要激素,JAZ蛋白是茉莉酸信号途径中重要的负调控因子。目前,巴西橡胶树中已报道7个JAZ基因。本研究结合Genebank中已有的橡胶c DNA序列和本实验室转录组测序数据,通过电子克隆的方法获得5个橡胶JAZ基因全长序列,分别命名为HbJAZ3、HbJAZ4、HbJAZ5、HbJAZ6和HbJAZ12,并通过RT-PCR技术成功扩增得到HbJAZ3、HbJA5、HbJAZ6、HbJAZ12等4个基因的编码区。最后采用定量RT-PCR技术分析发现,4个橡胶JAZ基因在橡胶不同组织中的表达有明显,以及其在伤诱导和茉莉酸处理后的差异表达,发现4个橡胶JAZ基因的表达都受到茉莉酸及创伤处理的影响,说明这些JAZ蛋白参与橡胶茉莉酸调控的创伤反应,本研究为阐明橡胶产排胶调控机理打下基础。
JA is an important hormone that regulates the traumatic reaction and gum production of rubber trees. JAZ is an important negative regulator of jasmonic acid signaling pathway. At present, seven JAZ genes have been reported in the Brazilian rubber tree. In this study, based on the existing rubber c DNA sequences in Genebank and the sequencing data of our laboratory transcriptome, five rubber JAZ full length sequences were obtained by electronic cloning and named as HbJAZ3, HbJAZ4, HbJAZ5, HbJAZ6 and HbJAZ12 respectively The coding regions of HbJAZ3, HbJA5, HbJAZ6 and HbJAZ12 were successfully amplified by RT-PCR. Finally, the quantitative RT-PCR analysis showed that the expression of four rubber JAZ genes in different tissues of rubber was obvious, as well as the differential expression of JAZ gene after injury induction and jasmonic acid treatment. The results showed that the expression of four rubber JAZ genes was affected by Jasmine Acid and trauma treatment, indicating that these JAZ proteins involved in rubber jasmonic acid regulation of the wound response, the study to clarify the mechanism of rubber gum production and lay the foundation.