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目的 :建立 HPL C梯度法测定血塞通胶囊中三七皂苷 R1、人参皂苷 Rg1、Rb1含量的方法。方法 :采用 HPL C法。色谱柱 :Shim-pack VP-ODS(150 MM× 4.6mm,5μm) ,流动相 :乙腈和水线性梯度洗脱 ,检测波长 :2 0 3 nm。结果 :三七皂苷 R1、人参皂苷 Rg1、Rb1线性范围分别为 6.3 5～ 3 1.75μg,6.9～ 3 4 .5μg,6.1～ 3 0 .5μg。该方法加样回收率 :三七皂苷R1为 96.9%、人参皂苷 Rg1为 98.4%、人参皂苷 Rb1为 96.3 % ,RSD分别为 2 .4% ,2 .6% ,1.8%。结论 :HPL C梯度洗脱法能将多种皂苷很好地分离检测 ,减少了误差 ,结果表明该方法准确、可靠 ,重现性好 ,可用于血塞通胶囊的含量测定。
Objective : To establish a method for determination of notoginsenoside R1 and ginsenoside Rg1 and Rb1 in Xuesaitong capsule by HPL C gradient method. Method : HPL C method was used. Column: Shim-pack VP-ODS (150 MM × 4.6mm, 5μm), mobile phase: linear gradient elution of acetonitrile and water, detection wavelength: 203 nm. Results: The linear ranges of notoginsenoside R1 and ginsenoside Rg1 and Rb1 were 6.3 5-31.75μg, 6.9-34.5μg, and 6.1-30. 5μg, respectively. The recoveries of the method were as follows: Panax notoginsenoside R1 was 96.9%, ginsenoside Rg1 was 98.4%, ginsenoside Rb1 was 96.3%, and RSD was 2.4%, 2.6%, and 1.8%, respectively. Conclusion : HPL C gradient elution method can separate and detect many kinds of saponins and reduce the error. The results show that the method is accurate, reliable, and has good reproducibility. It can be used for the determination of Xuesaitong capsules.