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目的 研究13 1I标记的抗TPS单克隆抗体 (13 1I TPS)及正常小鼠IgG(13 1I mIgG)在荷人乳腺癌裸鼠体内的放射免疫显像及生物学分布 ,为临床应用提供依据。方法 每只荷瘤鼠尾静脉注射标记抗体 3 7MBq/ 0 1ml,于注射后2 4、4 8、96、12 0小时行SPECT ,于 4 8、96、12 0小时分批处死 ,测定肿瘤、血液、肝、肺、等重要脏器的单位重量放射性比值(T/NT)及各组织摄取百分比 (%ID/g)。结果 13 1I TPS注射后 2 4～ 12 0小时内 ,SPECT可见阳性显像 ,阳性率为 5 8% ;瘤 /血比值在 12 0小时最高 ,达 2 5 1± 0 71。13 1I mIgG注射后在 2 4～ 12 0小时内未出现放射性浓聚呈全身性分布。结论 抗TPS单克隆抗体在荷人乳腺癌裸鼠体内 ,对过度表达TPS的乳腺癌具有亲合力 ,可望用于作乳腺癌诊断及预后判断的导向载体。
Objective To study the radioimmunoimaging and biological distribution of 131I-labeled anti-TPS monoclonal antibody (13I-TPS) and normal mouse IgG (131IIgIgG) in nude mice bearing human breast cancer and to provide basis for clinical application. METHODS: Tumor-bearing mice were injected with 3 7 MBq / ml of labeled antibody into the tail vein of each tumor-bearing mice at 24, 48, 96 and 120 hours after injection. SPECT was performed at 4, 8, 96 and 12 hours. Blood, liver, lung, and other important organs of the radioactive weight ratio (T / NT) and each tissue uptake percentage (% ID / g). RESULTS: SPECT showed a positive expression within 24 hours to 12 hours after injection of 1I TPS, with a positive rate of 58%. The tumor / blood ratio was the highest at 12 0 hours after the injection of 25 1 ± 0 71.13 1I mIgG In 2 4 ~ 12 0 hours did not appear radioactive concentration was systemic distribution. CONCLUSION: Anti-TPS monoclonal antibody has affinity for breast cancer overexpressing TPS in human breast cancer nude mice and is expected to be used as a guide vector for diagnosis and prognosis of breast cancer.