本文应用单纯疱疹病毒(HSV)糖蛋白D基因作为PCR引物,同时检测30例临床诊断单疱性角膜炎(HSK)患者的泪液及角膜上皮。用以比较两种取材的阳性结果为临床取材选最佳的方法。结果表明两种取材均检出单疱病毒DNA有较高的阳性率,二者阳性率比较经统计学处理无明显差异,但泪液取材较角膜上皮取材操作方便,损伤及副作用均小,因此,我们认为确认HSK,用PCR检测HSVDNA,泪液取材为首选方法。 In this paper, the herpes simplex virus (HSV) glycoprotein D gene was used as a PCR primer to detect the tear fluid and corneal epithelium in 30 patients with clinical herpes simplex keratitis (HSK). The positive results for the comparison of two materials are the best method of clinical selection. The results showed that both samples were detected positive herpes simplex virus DNA positive rate of positive rate compared with no statistically significant difference between the treatment, but the tear corneal epithelial material easy operation, injury and side effects are small, therefore, We think that confirming HSK, PCR detection of HSVDNA, tear fluid as the preferred method.