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目的 动态观察甲状腺激素受体 (TRs)mRNA在人脑发育过程中的表达变化。 方法 用RT PCR半定量法检测 3、5月龄人胎各脑区TRsmRNA表达变化。 结果 3、5月龄人胎大脑、小脑、海马、丘脑、脑干及脊髓均有TRsmRNA表达 ,其中 3月龄胎脑各脑区TRsmRNA表达以TRα1 、TRα2 为主 ,TRβ1 次之。 5月龄胎脑各脑区TRsmRNA表达中以TRβ1 、α2 为主 ,TRα1 次之。TRsmRNA在大脑、小脑、海马处表达相对丰富。实验中发现的与TRα2始终伴行的特异条带经测序证实 ,除在 371 4 12位氨基酸处比TRα2 少 4 2个氨基酸 ,余序列两者完全相同。 结论 在脑发育过程中 ,TRsmRNA呈时空性表达 ,参与调节CNS的分化、成熟。首次验证了人TRα3与TRα2 编码区的差异序列。
Objective To observe the changes of thyroid hormone receptor (TRs) mRNA expression during human brain development. Methods Semi-quantitative RT-PCR was used to detect the expression of TRsmRNA in various brain regions of 3, 5 months old human fetus. Results TRsmRNA expression was detected in human brain, cerebellum, hippocampus, thalamus, brainstem and spinal cord at 3, 5 month old. TRsm1, TRα2 and TRβ1 were the most frequent in brain regions of 3-month-old fetuses. The expression of TRsmRNA in brain regions of 5-month-old fetus was predominantly TRβ1 and α2, followed by TRα1. TRsmRNA is relatively abundant in brain, cerebellum and hippocampus. The specific bands always found in the experiment with TRα2 were confirmed by sequencing. Except for the 4242 amino acids at position 371 4 12 amino acids than TRα2, the remaining sequences were identical. Conclusion During the process of brain development, TRsmRNA is spatially expressed and involved in the regulation of CNS differentiation and maturation. For the first time, human TRα3 and TRα2 coding region of the difference sequence.