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目的:研究聚乙烯醇水凝胶与成骨细胞的生物相容性,为聚乙烯醇(Polyvinyl Alcohol,PVA)水凝胶应用于口腔软组织增量提供实验依据。方法:按照选定实验条件制备出PVA水凝胶块,将分切成合适大小的PVA水凝胶直接与成骨细胞(OB)联合培养,观察细胞的生长增殖情况,用MTT法检测PVA水凝胶对成骨细胞的毒性。结果:与PVA水凝胶直接接触的成骨细胞第1、2 d的相对增殖率接近99%,毒性分级为1级;第5 d的相对增殖率在99%以上,毒性分级为0级;第8、10 d的相对增殖率在90%以上,毒性分级为1级。PVA水凝胶组与阴性对照组间吸光度值无统计学差异(P>0.05),而PVA水凝胶组与空白对照组及阳性对照组的吸光度值比较,均有显著差异(P<0.05)。结论:PVA水凝胶凝胶具有良好的生物相容性,可用于口腔软组织增量。
OBJECTIVE: To study the biocompatibility of polyvinyl alcohol hydrogel with osteoblasts and provide experimental basis for the application of Polyvinyl Alcohol (PVA) hydrogel in oral soft tissue augmentation. Methods: PVA hydrogel blocks were prepared according to the selected experimental conditions. PVA hydrogels cut into appropriate sizes were directly cultured with osteoblasts (OB) to observe the growth and proliferation of cells. The activity of PVA hydrogel was detected by MTT assay Toxicity of gels to osteoblasts. Results: The relative proliferation rate of osteoblasts directly contacted with PVA hydrogel on day 1,2 was close to 99%, the toxicity grade was grade 1, the relative proliferation rate was above 99% on day 5, and the toxicity grade was 0 grade. The relative proliferation rate was above 90% on the 8th and 10th day, and the toxicity grade was 1 grade. There was no significant difference in absorbance between PVA hydrogel group and negative control group (P> 0.05), but there was significant difference between absorbance value of PVA hydrogel group and blank control group and positive control group (P <0.05) . Conclusion: PVA hydrogels have good biocompatibility and can be used for oral soft tissue augmentation.