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The absolute optical oscillator strength density spectra of nitric oxide in the energy region of 5.0-22.0 eV havebeen measured by a high-resolution fast-electron energy loss spectrometer. With the calculated results obtained by themultiscattering self-consistent-field method and channel characteristics, the strongly overlapped spectra in the energyregion of 7.5-9.3 eV have been analysed and the corresponding partially vibrationally resolved optical oscillator strengthshave been estimated from the experimental spectra.