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目的 :建立灵敏、可靠的梭曼光学异构体气相分析方法以测定小鼠皮下染毒后脑与膈肌中游离梭曼的浓度。方法 :大进样量气相色谱仪及手性毛细管柱实现梭曼 4个异构体的分离 ;用二异丙基氟磷酸酯作内标 ,测定小鼠皮下染毒后脑与膈肌中游离C(± )P(- )梭曼。结果 :梭曼 4个异构体在确定的气相条件下得到了较好的分离 ;C(± )P(- )梭曼在小鼠脑匀浆中及膈肌匀浆中 ,0 .5～ 10ng/ 2ml浓度范围的标准曲线线性关系良好 ,r >0 .999。日间、日内精密度在 10 %以内 ,提取方法的回收率为 5 3.6%～ 60 .2 %。用气相色谱分析方法考察了小鼠皮下染毒后 ,脑与膈肌中游离C(± )P( )梭曼的浓度 ,方法重复性好。结论 :大进样量气相色谱仪及手性毛细管柱测定小鼠皮下染毒后脑与膈肌中游离C(± )P(- )梭曼的浓度 ,方法灵敏、可靠 ,重复性好
OBJECTIVE: To establish a sensitive and reliable gas chromatographic method for soman optical isomers to determine the concentration of free soman in the brain and diaphragm after subcutaneous injection. METHODS: Four isomers of soman were isolated by gas chromatography and chiral capillary column. Diisopropylfluorophosphate was used as the internal standard to determine the content of free C ±) P (-) Soman. Results: The four isomers of soman were well separated under the definite gas phase conditions. The C (±) P (-) soman in the brain homogenate of mouse and in the diaphragm homogenate, 0.5 ~ 10ng / 2ml concentration range of the standard curve of a good linear relationship, r> 0.999. During the day and the day, the precision was within 10%, and the recovery rate of the extraction method was 53.6% ~ 60.2%. The concentration of free C (±) P () soman in the brain and diaphragm was investigated by gas chromatography after the mice were subcutaneously exposed. The method was reproducible. Conclusion: The concentration of free C (±) P (-) soman in the brain and diaphragm after subcutaneous injection in mice was determined by gas chromatography and chiral capillary column. The method was sensitive, reliable and reproducible