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Chitin is a kind of seemly material to match PLLA for a scaffold, which may create an appropriate environment for the regeneration of tissues. In this study, we evaluated hydrophilicity of a new nano-hydroxyapatite/collagen/PLLA (nHACP) scaffold optimized by chitin fibres for bone tissue engineering. The results show that with the CF content increase, hydrophilicity of nHACP/CF increases, which reflects from the side that the addition of the chitin fibres can improve the cytocompatibility of the nHACP. Moreover, crosslink does not take significant influences on the material hydrophilicity. The results suggest that nHACP/CF with the crosslink should be a kind of potential appropriate scaffold for tissue engineering.