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目的探讨维生素D_3对溃疡性结肠炎(ulcerative colitis,UC)大鼠肠黏膜Toll样受体2(Toll-like receptor 2,TLR2)和髓样分化蛋白2(myeloid differentiation protein 2,MD2)及CD14表达的影响,及辅助治疗UC的可能机制。方法 30只SD大鼠随机分为正常对照组、模型组和维生素D_3干预组各10只。模型组和维生素D_3干预组将质量分数5%2,4,6-三硝基苯磺酸与体积分数50%乙醇等体积混合后以0.4mL/100g剂量缓慢注入大鼠肠道制备UC模型,正常对照组以等量生理盐水灌肠。维生素D_3干预组在造模成功后第10天起给予胆维丁乳原液1 875u灌胃,模型组和正常对照组给予等剂量生理盐水灌胃,均1次/d,连续10d;比较3组大鼠一般情况及维生素D_3干预第10天疾病活动指数(disease activity index,DAI)评分;维生素D_3干预第10天处死大鼠,取结肠黏膜进行组织病理学评分(histopathological score,HPS),采用免疫组织化学法检测3组结肠黏膜组织TLR2、MD2和CD14的表达情况。结果维生素D_3干预第10天,模型组与维生素D_3干预组DAI[(8.07±1.87)、(5.15±2.40)分]、HPS评分[(14.33±0.97)、(10.01±2.23)分]高于正常对照组(0、0分)(P<0.05),维生素D_3干预组低于模型组(P<0.05);TLR2、MD2和CD14均在肠黏膜上皮细胞细胞质、细胞核中着色;结肠黏膜组织TLR2、MD2和CD14阳性表达率在模型组(90%、80%、100%),维生素D_3干预组(80%、50%、50%)均高于正常对照组(0、0、0),且模型组高于维生素D_3干预组(P<0.05)。结论结肠黏膜组织中TLR2、MD2和CD14高表达可能与UC发病有关,维生素D_3可通过抑制TLR2、MD2和CD14表达减轻UC大鼠的肠道炎症反应,发挥辅助治疗UC的作用。
Objective To investigate the effect of vitamin D_3 on the expression of toll like receptor 2 (TLR2) and myeloid differentiation protein 2 (MD2) and CD14 in intestinal mucosa of rats with ulcerative colitis (UC) And the possible mechanism of adjuvant treatment of UC. Methods Thirty SD rats were randomly divided into normal control group, model group and vitamin D 3 intervention group. Model group and vitamin D_3 intervention group, the mass fraction of 5% 2,4,6-trinitrobenzene sulfonic acid and the volume fraction of 50% ethanol mixed volume of 0.4mL / 100g dose slowly into the rat intestine preparation UC model, The normal control group with the same amount of saline enema. Vitamin D_3 intervention group was given the first 10 days after the success of gallbladder solution of 1 875u gavage, the model group and normal control group were given equal dose of saline, were 1 / d, continuous 10d; compared 3 groups The rats were sacrificed on the 10th day after intervention with vitamin D_3, and the rats were sacrificed on the 10th day after intervention with vitamin D_3. The histopathological score (HPS) The histochemical method was used to detect the expression of TLR2, MD2 and CD14 in the three groups of colonic mucosa. Results On the 10th day after intervention with vitamin D 3, DAI (8.07 ± 1.87) and (5.15 ± 2.40) points in model group and vitamin D 3 intervention group were significantly higher than those in normal control group [(14.33 ± 0.97) and (10.01 ± 2.23) points, respectively] The levels of TLR2, MD2 and CD14 in colorectal mucosa epithelial cells in the control group (0, 0) (P <0.05) The positive expression rates of MD2 and CD14 in model group (90%, 80%, 100%) and vitamin D 3 intervention group (80%, 50%, 50%) were higher than those in normal control group (0,0,0) Group than vitamin D 3 intervention group (P <0.05). Conclusion The high expression of TLR2, MD2 and CD14 in colonic mucosa may be related to the pathogenesis of UC. Vitamin D 3 can relieve the intestinal inflammatory response in UC rats by inhibiting the expression of TLR2, MD2 and CD14 and play a role in the adjuvant treatment of UC.