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利用两对SSR引物对两个基因座Pi4B和Pi4G进行了PCR扩增,测定了中国部分地区66个致病疫霉Phyophthora infestans(马铃薯晚疫病菌)菌株和2个参考菌株的SSR基因型,并对菌株的基因型进行了鉴定和命名。在被测定的66个致病疫霉菌株中,共产生了7种SSR基因型D-03,D-05,D-06,G-02,H-01,F-01和F-06,其中F-06为本研究新命名的基因型。F-01基因型菌株53个,占总菌株数目的80.3%,该基因型为中国致病疫霉的优势基因型。在对两个基因座Pi4B和Pi4G产生的等位基因统计分析发现基因座Pi4B产生的多样性比Pi4G高。对SSR数据揭示的河北、黑龙江和云南3个不同省份致病疫霉遗传多样性的比较发现,河北省和黑龙江省致病疫霉遗传多样性几乎相同,然而与云南省致病疫霉有较大的遗传差异。此外,发现致病疫霉SSR基因型与其对甲霜灵抗性无相关性。
Two pairs of SSR primers were used to amplify the two loci, Pi4B and Pi4G, and SSR genotypes of 66 Phyophthora infestans strains and two reference strains in some areas of China were determined. The strain’s genotypes were identified and named. Seven SSR genotypes D-03, D-05, D-06, G-02, H-01, F-01 and F-06 were produced in the 66 P. infestans strains tested F-06 is the newly named genotype in this study. There were 53 F-01 genotypes, accounting for 80.3% of the total number of strains. The genotype was the dominant genotype of Phytophthora infestans in China. Statistical analysis of alleles at the two loci Pi4B and Pi4G revealed that loci Pi4B produced a greater diversity than Pi4G. Comparison of the genetic diversity of Phytophthora infestans revealed in three different provinces of Hebei, Heilongjiang and Yunnan Province revealed by SSR data showed that the genetic diversity of Phytophthora infestans in Hebei Province and Heilongjiang Province was almost the same, but compared with that of Phytophthora infestans in Yunnan Province Big genetic differences. In addition, the SSR genotype of P. infestans was found to be unrelated to its resistance to metalaxyl.