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目的建立一株既保存细胞的功能特性，又容易长期和大量繁殖的标准肌腱细胞。方法采用电穿孔法，在９６０μＦ、４００Ｖ条件下将国外构建的质粒ｐｔｓＡ５８Ｈ导入肌腱细胞。结果经潮霉素Ｂ严格筛选，挑选出了阳性克隆。结论该转化成功的为肌腱细胞标准细胞株建立了转化模型。
Objective To establish a standard tendon cell that preserves the functional properties of cells and is prone to long-term and large-scale reproduction. Methods The plasmid ptsA58H constructed abroad was introduced into tendon cells by electroporation at 960μF and 400V. The results of strict selection by hygromycin B, picked out the positive clones. CONCLUSIONS: A successful transformation model was established for a standard cell line of tendon cells.