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作者用自制的反应池与国产测氧仪等配套建立了微粒体脂质过氧化体系中耗氧量的测定方法,并观察了铁、铜、锌对耗氧量的影响.玻璃反应池的内池置反应液并接氧电极与CY-2型测氧仪相连,外池充满循环恒温水.方法精密度测定,加入5μmol/L铜时重复7次测耗氧量,x±S为0.975±0.030μmol/(gprotein.min),CV为3.01％.实验结果表明:在大鼠肝微粒体NADPH-ADP/Fe~(2+)系统中,铁呈现对脂质过氧化的刺激作用,而锌、铜则表现为抗氧化作用,即对耗氧量的抑制作用.0,0.5、1.0,1.5mmol/L铁的刺激率(％)分别为1.7±1.1,89.1±3.3,108.7±7.5;而50,200.500μmol/L锌的抑制率(％)分别为20.8±4.5,40.6±6.3、59.2±1.4;5,50,100μmol/L铜的抑制率(%)分别为27.6±14.9.39.8±0.8、6.6±3.4
The authors used homemade reaction cell and homemade oxygen measuring instrument to establish the method of measuring oxygen consumption in microsomal lipid peroxidation system and observed the influence of iron, copper and zinc on oxygen consumption. The reaction solution was put in the pool and the oxygen electrode was connected with the oxygen measuring instrument CY-2, and the outer tank was filled with circulating constant temperature water.METHODS The precision was measured, the oxygen consumption was measured 7 times after adding 5μmol / L copper, x ± S was 0.975 ± 0.030 μmol / gprotein.min and the CV was 3.01% .Experimental results showed that iron showed a stimulatory effect on lipid peroxidation in rat liver microsome NADPH-ADP / Fe 2+ system, whereas zinc, Copper showed an anti-oxidant effect on the oxygen consumption, and the rates of irritation (%) of iron at 0, 0.5, 1.0 and 1.5 mmol / L were 1.7 ± 1.1, 89.1 ± 3.3 and 108.7 ± 7.5 respectively; μmol / L zinc were 20.8 ± 4.5, 40.6 ± 6.3 and 59.2 ± 1.4, respectively; the inhibitory rates (%) of copper at 5, 50 and 100μmol / L were 27.6 ± 14.9.39.8 ± 0.8,6.6 ± 3.4