目的研究菘蓝不同部位干品和鲜品提取物的抑菌作用,为菘蓝的进一步开发利用提供理论依据。方法采用水提醇沉提取精制,活性炭脱色;采用试管二倍稀释法统计最小抑菌浓度(MIC)采用平板二倍稀释法统计最小杀菌浓度(MBC)。结果板蓝根干燥药材和新鲜药材对大肠杆菌MIC分别为0.25 g/m L、0.5 g/m L,MBC分别为1.0 g/m L、2.0 g/m L;对金黄色葡萄球菌MIC分别为0.25 g/m L、0.25 g/m L,MBC=1.0 g/m L、2.0 g/m L;对绿脓杆菌MIC分别为0.50 g/m L、1.0 g/m L,MBC=1 g/m L,2.0 g/m L。大青叶干燥药材和新鲜药材对大肠杆菌MIC分别为0.5 g/m L、4.0 g/m L;对金黄色葡萄球菌MIC为0.5 g/m L、2.0 g/m L;对绿脓杆菌MIC为0.5 g/m L、2.0 g/m L;大青叶干品和鲜品提取液实验浓度未测出上述三种细菌的MBC,仅呈现出一定的抗菌趋势。结论菘蓝根和叶对三种菌株均有一定的抑制或杀灭作用,按照干品和鲜品质量换算,抗菌效价鲜品强于干品;入药部位比较,板蓝根优于大青叶。 OBJECTIVE To study the bacteriostasis of dry and fresh extracts from different parts of Isatis indigotica so as to provide a theoretical basis for the further development and utilization of radix islani. Methods Extraction and purification by water extraction and alcohol precipitation and decolorization of activated carbon were performed. The minimum inhibitory concentration (MIC) was determined by the double dilution method of test tube and the minimum bactericidal concentration (MBC) was calculated by the plate double dilution method. Results The results showed that the MICs of Radix isatidis were 0.25 g / m L and 0.5 g / m L, MBC was 1.0 g / m L and 2.0 g / m L, respectively. The MIC values ​​of S. aureus were 0.25 g / / mL, MBC = 1.0 g / mL, 2.0 g / mL; for P. aeruginosa MICs were 0.50 g / mL, 1.0 mL / L, MBC = , 2.0 g / m L. The results showed that the MIC values ​​of the dried medicinal materials and fresh medicinal materials were 0.5 g / m L and 4.0 g / m L for Escherichia coli, 0.5 g / m L and 2.0 g / m L for Staphylococcus aureus respectively, 0.5 g / m L and 2.0 g / m L. The MBC of the above three kinds of bacteria was not detected in the experimental concentration of the dry matter and the fresh extract of Folium Isatidis, and showed only a certain antibacterial tendency. Conclusion Allium saponins and leaves have certain inhibition or killing effects on the three strains. According to the quality of dried and fresh products, the fresh antibacterial products are stronger than the dry products.