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目的了解南宁市婴幼儿散发性腹泻病例中诺如病毒(Norovirus,NV)基因Ⅱ组(GenogroupⅡ,GⅡ)4型变异株的组成和流行特点。方法采集2010年1月~2011年12月南宁市某医院门诊354例婴幼儿腹泻病例的粪便标本,应用实时荧光逆转录-聚合酶链反应检测NV核酸及其基因组,阳性标本进行核苷酸序列测定和遗传进化分析。结果 354份标本中,NV核酸检测阳性101份,阳性率28.5%,全部属于GⅡ。对测序成功的84株NV的核苷酸序列进行分析,77.4%属于GⅡ.4型(65株),其余为GⅡ.2型(7株)、GⅡ.14型(4株)、GⅡ.7型(3株)、GⅡ.3型(2株)、GⅡ.6型(2株)和GⅡ.12型(1株)。65株GⅡ.4型中,60株(92.3%)为2006b变异株,5株(7.7%)为2010变异株。结论 GⅡ.4型是南宁市婴幼儿中NV所致散发性腹泻的最主要基因型,其中2006b变异株占优势。首次在本地区检测到2010变异株,且该毒株的流行呈上升趋势。有必要加强监测,以及时掌握GⅡ.4型变异株的流行变迁。
Objective To understand the composition and epidemiological characteristics of Genogroup Ⅱ (GⅡ) 4 variant of Norovirus (NV) gene in infants with infant and childhood diarrhea in Nanning. Methods A total of 354 cases of infantile diarrhea were collected from a hospital in Nanning City from January 2010 to December 2011. Real-time fluorescence reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the presence of NV nucleic acid and its genome. The positive samples were sequenced for nucleotide sequence Assay and Genetic Analysis. Results Of the 354 samples, 101 were positive for NV nucleic acid, the positive rate was 28.5%, all of which belonged to GⅡ. The nucleotide sequences of 84 NVs sequenced were analyzed, 77.4% belonged to GⅡ.4 type (65 strains), the rest were GⅡ.2 type (7 strains), GⅡ.14 type (4 strains), GⅡ.7 (3 strains), GⅡ.3 type (2 strains), GⅡ.6 type (2 strains) and GⅡ.12 type (1 strain). Of the 65 GⅡ.4 types, 60 (92.3%) were 2006b and 5 (7.7%) were 2010 variants. Conclusion GⅡ.4 type is the most important genotype of sporadic diarrhea caused by NV in infants and young children in Nanning. The variant of 2006b is dominant. For the first time in 2010 in the region detected mutants, and the strain of the epidemic is on the rise. There is a need to step up monitoring to keep abreast of the epidemics of GII.4 mutants.