BACKGROUND:Recently,many investigators have tried to use natural biomaterials,such as,artery,vein,decalcified bone,etc.,as conduits for nerve repair.However,immunological rejection of conduits made of natural biomaterials limits their application.Therefore,it is essential to identify more suitable types of biomaterials.OBJECTIVE:To observe the characteristics of a bioengineering processing method using venous conduit as a stent for repairing facial nerve injury.DESIGN:A controlled observational experiment.SETTING:Animal Laboratories of the Third Hospital Affiliated to Sun Yat-sen University and the 157 Hospital.MATERIALS:Thirty-three male New Zealand rabbits of pure breed,weighing 1.5 to 2.0 kg,were provided by Medical Experimental Animal Room of Sun Yat-sen University.The protocol was carried out in accordance with animal ethics guidelines for the use and care of animals.Venous conduits and autogenous nerves were transplanted into the left and right cheeks,respectively.Eleven animals were chosen for anatomical observations at 5,10 and 15 weeks after surgery.METHODS:This experiment was carried out in the Animal Laboratories of the Third Hospital Affiliated to Sun Yat-sen University and the 157 Hospital between May and November 2006.After animals were anesthetized,15 mm of retromandibular vein was harvested for preparing a venous conduit.Approximately 3 cm of low buccal branch of facial nerve was exposed.A segment of 1.2 cm nerve was resected from the middle,and a gap of 1.5 cm formed due to bilateral retraction.The prepared venous conduit of 1.5 cm was sutured to the outer membrane of the severed ends of the nerve.Muscle and skin were sutured layer by layer.Using the same above-mentioned method,the low buccal branch of right autogenous facial nerve was resected,and the left facial nerve segment from the same animal was transplanted using end-to-end neurorrhaphy for control.MAIN OUTCOME MEASURES:①Post-operatively,food intake,vibrissae activity and wound healing of each animal were observed daily.② Animals were anesthetized at 5,10 and 15 weeks after operation for observing the structural change of the venous conduit,the appearance of regenerated nerve,and the relationship between conduit and peripheral muscle tissue.③The action potential and latency of bilateral nerves of animals were measured by electrophysiologic examination,and nerve conduction velocity was calculated.④Neural myelination and neurite growth were observed by histological staining using an optical microscope.RESULTS:Thirty-three New Zealand rabbits were involved in the final analysis.①mmediately following the operation,vibrissae activity and orbicularis otis muscle activity of the upper lip on venous conduit side were more prominent,and their amplitudes of movement were larger as compared with autogenous nerve side.②At postoperative 10 weeks,by visual inspection,we found that on the venous conduit side,the venous conduit exhibited membrane structure which encased regenerated nerve.Regenerated nerve adhered to the muscle edge of orbicularis oris muscle.Muscle and nerve could be separated with a forceps.The muscle of musculus orbicularis oris of rabbit was darker and thicker as compared with autogenous nerve side.After the venous conduit was longitudinally split,the regenerated nerve and nerves at two the severed ends were connected together.When compared with postoperative 5 weeks,the connected nerve was thickened,texture was tough and its middle part was thicker than its two ends.On the autogenous nerve side,the regenerated nerve stem was enwrapped by scar tissue.It was bulky and adhered to peripheral muscle.Its neural profile structure was unclear.The two stomas were obviously enlarged.③At postoperative 10 weeks and 15 weeks,nerve action potentials could be elicited from both the venous conduit and autologous nerve side.The mean nerve conduction velocity on the venous conduit side was greater than that of the autologous nerve side.④At postoperative 10 weeks,using histochemical staining,it was found that in the venous conduit,regenerated medullated nerve fibers were densely distributed,with well split facial nerve structure,while on the autologous nerve side,nerve fibers were sparsely scattered,with immature medullated nerve structure.CONCLUSION:Biological natural venous conduit processed by bioengineering technology overcomes the tissue inflammatory reactions and connective tissue reactions caused by natural biomaterials.It is more conducive to promote neural regeneration and functional recovery than autologous nerve transplantation.