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目的 :研究白桦三萜类物质 (TBP )体内抗肿瘤作用及其对荷瘤小鼠免疫功能的影响。方法 :利用小鼠体内移植瘤模型 ,测定TBP对小鼠黑色素瘤B16、肉瘤S180、Lewis肺癌和艾氏腹水癌等肿瘤的抑瘤率。采用放射性掺入法检测ConA诱导的脾细胞增殖反应 ;放射性释放法检测巨噬细胞细胞毒活性 ;中性红释放法检测TNF和NK细胞活性。结果 :TBP对各瘤株的抑瘤率均达到 30 %以上 ,对黑色素瘤B16的效果最好 ,抑瘤率为 5 1 4%。TBP对ConA诱导的增殖反应和NK细胞活性无明显影响 ,但可促进巨噬细胞和脾细胞分泌TNF ,增加巨噬细胞的细胞毒活性。结论 :TBP具有良好的抗肿瘤作用 ,增强机体的非特异性免疫功能是其抗肿瘤机制之一
Objective: To study the anti-tumor effect of birch (BBP) in vivo and its effect on immune function of tumor-bearing mice. METHODS: The mouse tumor xenografts in vivo were used to determine the tumor inhibition rates of TBP in mice with melanoma B16, sarcoma S180, Lewis lung cancer and Ehrlich ascites carcinoma. The ConA-induced proliferation of spleen cells was detected by radioactive incorporation method, macrophage cytotoxic activity was detected by radioactive release method, and TNF and NK cell activity was measured by neutral red release method. RESULTS: The tumor inhibition rate of TBP on tumor strains reached more than 30%, the best effect on melanoma B16, and the tumor inhibition rate was 51.4%. TBP has no significant effect on ConA-induced proliferation and NK cell activity, but it can promote macrophages and splenocytes to secrete TNF and increase macrophage cytotoxic activity. Conclusion: TBP has a good anti-tumor effect. Enhancing the body’s non-specific immune function is one of its anti-tumor mechanisms.