川芎(Ligusticum chuanxiong Hort.)是常用中药,具有活血行气,祛风止痛作用,含阿魏酸、挥发油等成分.本文报道川芎愈伤组织的诱导和悬浮培养,并用薄层层析法分析了培养物的有效成分. 材料与方法组织培养取川芎新长出1～2片真叶的幼嫩茎段为外植体,常规消毒.培养基为MS,蔗糖3%,琼脂0.6%,pH5.8.分别附加激素2,4-D 2(mg/L,下同),2,4-D_2+6-BA Chuanxiong (Ligusticum chuanxiong Hort.) is a commonly used traditional Chinese medicine. It has the functions of promoting blood circulation, removing wind and relieving pain, containing ferulic acid, volatile oil and other components. This article reports the induction and suspension culture of callus of Ligusticum chuanxiong Hort. It was analyzed by thin layer chromatography. The active ingredients of the culture. Materials and methods Tissue culture Chuanxiong new young stems growing from 1 to 2 true leaves as explants, conventional disinfection. Medium MS, sucrose 3%, agar 0.6%, pH5. 8. Additional hormones 2,4-D 2 (mg/L, the same below), 2,4-D_2+6-BA