α-亚麻油酸(α-linolenic acid,ALA)对防治脂类代谢失调而引起的肥胖、心血管疾病、高血脂、高血压等慢性疾病具有重要的生理功能。然而,大部分油料作物种子ALA的含量都很低,提高种子ALA的含量一直是油料作物品质改良的重点内容之一。星油藤(Plukenetia volubilis)种子油中富含ALA,是一种具有重要开发前景的油料植物,解析其种子高效累积ALA的分子机理不仅能为星油藤的遗传改良提供理论依据,而且能够为提高油料作物种子ALA含量的遗传育种提供理论参考。前期的研究发现星油藤种子高效累积ALA主要是由于关键酶基因Pv FAD3高度表达的结果。为了进一步理解星油藤种子在发育过程中Pv FAD3的被调控机理,本研究利用染色体步移法克隆了Pv FAD3的启动子,并且利用生物信息学分析、鉴别了启动子潜在的关键调控元件,并重点围绕可能调控Pv FAD3基因表达的b ZIP124转录因子,通过酵母单杂交和烟草叶片启动子激活实验,验证了Pv FAD3的表达受b ZIP124转录因子的调控。该研究为理解植物种子高效累积ALA的分子机理提供了新的证据。 Alpha-linolenic acid (ALA) has important physiological functions for the prevention and treatment of chronic diseases such as obesity, cardiovascular diseases, hyperlipidemia and hypertension caused by dyslipidemia. However, the content of ALA in most oilseed crops is very low. Increasing the content of ALA in the seeds has always been one of the important contents of improving the quality of oilseed crops. The seed oil of Plukenetia volubilis is rich in ALA, which is an oil plant with important development prospects. Analyzing the molecular mechanism of efficient accumulation of ALA in seeds of Plukenetia volubilis not only provides a theoretical basis for the genetic improvement of Astragalus sinicus, Provide a theoretical reference for improving the ALA content of oilseed crop by genetic breeding. The previous study found that the efficient accumulation of ALA in C. oleifera seeds was mainly due to the high expression of Pv FAD3, a key enzyme gene. In order to further understand the regulation mechanism of Pv FAD3 during the development of A. capitatum, we used the chromosome walking method to clone the promoter of Pv FAD3. By using bioinformatics analysis, we identified the potential key regulatory elements of promoter, And focused on the b ZIP124 transcription factor that may regulate the expression of Pv FAD3 gene. Through the yeast single hybridization and tobacco leaf promoter activation experiments, we verified that the expression of Pv FAD3 is regulated by b ZIP124 transcription factor. This study provides new evidence for the understanding of the molecular mechanism by which plant seeds efficiently accumulate ALA.