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高密度脂蛋白结合蛋白(high density lipoprotein binding protein,HBP)在动物的脂肪代谢中发挥重要的作用。脂类是鱼类的重要能源物质,尤其是那些对糖类利用率很低的肉食性鱼类。HBP基因的单核苷酸多态性(single nucleotide polymorphism,SNP)可能会影响脂肪代谢,而对大口黑鲈的生长性状产生影响,研究其SNP与生长性状的相关性可为其分子标记辅助选择提供候选标记。本研究根据大口黑鲈(Micropterus salmoides)EST-SNP库中HBP基因的序列(GenBank accession No.KF652241),用直接测序法在HBP基因3’非编码区获得了3个SNPs位点:H1(G+2782T),H2(T+2817C)和H3(G+2857A);随机选择同批繁殖、同塘养殖的165尾大口黑鲈样本,对3个SNPs位点用SnaPshot方法进行检测和分型,分型结果用软件Popgene32进行遗传结构分析;运用软件spss17.0的一般线性模型(general linear model,GLM)对获得的不同基因型与生长性状进行关联分析。结果表明,3个位点均处于哈温平衡,其中H1和H2位点组成了两种单倍型(A和B),观测到3种基因型(AA、AB和BB);H1、H2和H3共组成6种双倍型(D1、D2、D3、D4、D5和D6)。不同基因型与生长性状的相关性分析显示,基因型BB在体质量和全长形态指标显著高于基因型AB(P<0.05),双倍型D6在体质量和全长形态指标显著高于双倍型D4(P<0.05)。本研究在大口黑鲈HBP基因3’非编码区获得了与生长性状相关的SNPs标记,可应用于大口黑鲈分子标记辅助选择育种。
High density lipoprotein binding protein (HBP) plays an important role in animal fat metabolism. Lipids are important energy sources for fish, especially those carnivores that have low rates of carbohydrate utilization. The single nucleotide polymorphism (SNP) of HBP gene may affect fat metabolism, but has an impact on the growth traits of largemouth bass, and the correlation between SNP and growth traits may be its molecular marker-assisted selection Provide candidate tags. Based on the HBP gene sequence (GenBank accession No.KF652241) in the EST-SNP library of Micropterus salmoides, we obtained three SNPs in the 3 ’non-coding region of HBP by direct sequencing: H1 (G + 2782T), H2 (T + 2817C) and H3 (G + 2857A). A total of 165 largemouth bass samples from the same breed and pond were randomly selected and detected by SnaPshot method. The genotyping results were analyzed by Popgene32 software. The genotypes and growth traits obtained were analyzed by general linear model (GLM) software spss17.0. The results showed that all three loci were in equilibrium with HAV, in which two haplotypes (A and B) were composed of H1 and H2 loci and three genotypes (AA, AB and BB) were observed; H1, H2 and H3 forms six haplotypes (D1, D2, D3, D4, D5 and D6). The correlation analysis of different genotypes and growth traits showed that the body mass and full-length morphological parameters of genotype BB were significantly higher than those of genotype AB (P <0.05), while the body weight and full- Doubling D4 (P <0.05). In this study, SNPs markers related to the growth traits were obtained in the 3 ’non-coding region of the HBP gene of largemouth bass, and could be applied to molecular marker-assisted selection breeding of largemouth bass.