通过研究阿糖尿苷与人血清白蛋白(HSA)体系的三维荧光光谱,证明两者可以发生相互作用,并导致HSA的微环境和构象发生变化。考查了Δλ、加入顺序、离子强度等因素对该体系同步荧光强度的影响,选取阿糖尿苷测定生物样品中蛋白含量的最佳实验条件。体系的同步荧光强度与HSA的浓度在0.04～4.0×10-6mol/L范围内呈良好的线性关系,此法的检测限可达0.75 mg/L,线性相关系数为0.9990。在此基础上,以阿糖尿苷为分子探针,测定了人血清、唾液和尿样中的蛋白质含量,回收率在97.3%～104.5%之间。 By studying the three-dimensional fluorescence spectra of arabinoside and human serum albumin (HSA) system, it is proved that the two can interact with each other and lead to the change of microenvironment and conformation of HSA. The effects of Δλ, order of addition, ionic strength and other factors on the synchronous fluorescence intensity of the system were investigated. The optimal experimental conditions for the determination of protein content in biological samples were selected. The synergistic fluorescence intensity of HSA was linear with the concentration of HSA in the range of 0.04-4.0 × 10-6mol / L. The detection limit of this method was up to 0.75 mg / L and the linear correlation coefficient was 0.9990. On this basis, the protein content of human serum, saliva and urine samples was measured with arabinouridine as a molecular probe, and the recoveries ranged from 97.3% to 104.5%.