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用纯化的日本血吸虫成虫31/32KD抗原建立的BA-ELISA系统检测慢性血吸虫病患者治疗前、后血清中的IgG1,IgG3和IgG4抗体水平;同时用常规ELISA检测IgG和IgM特异抗体。结果表明:各类抗体均未见假阳性,亦未与肝吸虫病和肺吸虫病患者血清出现交叉阳性。提示IgG1和IgG4抗体的敏感性和特异性高,具有诊断和疗效考核的价值。
The serum level of IgG1, IgG3 and IgG4 antibody in patients with chronic schistosomiasis was detected by using the purified BA-ELISA system of 31 / 32KD antigen of Schistosoma japonicum. IgG and IgM specific antibodies were detected by routine ELISA. The results showed that all kinds of antibodies did not show any false positive, nor did they cross positive with the serums of patients with paragonimiasis and paragonimiasis. Prompt IgG1 and IgG4 antibody sensitivity and specificity, with diagnostic and therapeutic evaluation of the value.