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近年来,我国淋病患者有增多的趋势。为寻求一种敏感、特异、简单、快速的检查方法,本工作自制成鼠抗淋球菌(GC)抗体,建立了淋球菌-酶免疫法检查(GC-EIA)。并用以检查临床上不同类型的感染者和部分健康体检者,且对本法的灵敏性和特异性,以及实验中的一些影响因素等作了探讨,现报道如下。 材料和方法 一、材料: (一)GC来源;自淋病患者尿道分泌物及阴道分泌物中培养分离,并经革兰氏染色、生化鉴定确证。取混合菌株的培养物,用
In recent years, there is an increasing trend of gonorrhea patients in our country. In order to find a sensitive, specific, simple and rapid test method, this work made a mouse anti-gonococcal (GC) antibodies, the establishment of the Neisseria gonorrhoeae - enzyme immunoassay (GC-EIA). And used to examine different clinical types of infected persons and part of the health examination, and the sensitivity and specificity of this Act, as well as some of the experimental factors were discussed, are reported below. Materials and methods First, the material: (a) GC source; from the gonococcal urethral secretions and vaginal secretions were cultured and isolated, and Gram stain, biochemical identification confirmed. Take mixed strains of culture, with