A simple sensitive spectrofluorimetric method was developed for the determination of amoxicillin.Amoxicillin in the presence of formaldehyde produces a fluorescent pyrazine derivative, whose fluorescence intensity can be enhanced by forming an inclusion complex with β-cyclodextrin. Optimum conditions for the determination of amoxicillin were also investigated. Fluorimetric detection was carried out at λex=365 nm and λex=445 nm. The linear range of this method for the determination of amoxicillin is 0.1-10 mg/L and the limit of detection is 0.03 mg/L (IF=102.16p-9.6, r=0.9994). The developed method is practical and can be successfully applied to determine the content of amoxicillin in capsules and tablets with satisfactory results.