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以北玄参的无菌苗叶片为外植体,直接诱导不定芽并得到再生植株,建立了北玄参叶片直接再生体系。结果表明,北玄参种子直接用0.1%Hg Cl2灭菌6 min就能达到良好的消毒效果,污染率为4.95%,种子萌发率为81.49%;诱导北玄参叶片再生不定芽最佳培养基为MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1,诱导率可达100%;芽增殖培养基为MS+6-BA 0.2 mg·L-1+NAA 0.2 mg·L-1,增殖倍数为9.13;诱导生根的培养基为1/4MS+IBA 0.2 mg·L-1,生根率100%,平均生根数为11.33;再生植株移栽的成活率为86.96%。
Radix Aconiti Kusnezoffii as a explant, the direct induction of adventitious buds and regenerated plants, the establishment of a direct regeneration of the North Scrophulariaceae leaves system. The results showed that the seeds of Radix Scrophulariae could be disinfected with 0.1% Hg Cl2 directly for 6 min, with a contamination rate of 4.95% and a seed germination rate of 81.49%. The optimum culture medium of Radix And the induction rate was 100% for MS + 6-BA 0.5 mg · L-1 + NAA 0.2 mg · L-1. The bud proliferation medium was MS + 6-BA 0.2 mg · L -1 + NAA 0.2 mg · L -1, and the multiplication ratio was 9.13. The medium for inducing rooting was 1 / 4MS + IBA 0.2 mg · L-1, the rooting rate was 100% and the average rooting number was 11.33. The survival rate of regenerated plants was 86.96%.