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为评价HBsAg阴性的一系列酒精性肝病患者中,酒精性肝病与“不显性”HBV和HCV感染间的关系,作者用聚合酶链反应(PCR)方法,通过血清HBV PCR和HCV逆转录酶“一管、套式”PCR法检测,评估了HBV和HCV感染的可能性。将HCVPCR法与常用的两种抗-HCV检测法:第2代重组兔疫印迹法(RIBA_2)和第2代酶联免疫吸附试验(ELISA_2)进行比较,以评价它们的符合程度。
To evaluate the relationship between alcoholic liver disease and “nonobvious” HBV and HCV infection in a series of HBeAg-negative patients with alcoholic liver disease, the authors used polymerase chain reaction (PCR), serum HBV PCR and HCV reverse transcriptase “One tube, nested” PCR assay to assess the likelihood of HBV and HCV infection. The HCVPCR method was compared with two commonly used anti-HCV assays: the second generation recombinant rabbit immunoblot (RIBA_2) and the second generation enzyme-linked immunosorbent assay (ELISA_2) to evaluate their compliance.