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野生干巴菌(Thelephora ganbajun)产量有限,探索其人工或半人工栽培方法自然成为人们关注的问题。而干巴菌菌种的分离则是研究其生活史及人工栽培的前提条件之一,对干巴菌繁殖具有重要意义。本工作在于探索分离干巴菌菌种的合适方法。 1 试验材料 1.1 子实体 采集于昆明小哨的成熟新鲜子实体(灰白或褐色,帚状小分枝明显,顶端小枝片达5cm以上)和幼嫩子实体(鲜黄色,未见珊瑚状分枝,直径0.5～2cm)。 1.2 培养基 斜面培养基为马铃薯20g,葡萄糖20g,硫酸镁0.5g,磷酸二氢钾0.25g,硫胺素30μg,琼脂20g,水1000mL,121℃灭菌30分钟制成斜面。A培养基:将上述灭菌培养基熔化,稍冷却后在无菌室内加链霉素50μg/mL,摇匀,倒入灭菌培养皿,每皿1O～15mL,厚
The production of Thelephora ganbajun is limited, and exploring artificial or semi-artificial cultivation has naturally become a matter of concern. The separation of the genus Candidasis is one of the prerequisites for the study of their life history and artificial cultivation, which is of great significance for the growth of the genus. This work is to explore the appropriate method of isolation of strains of Candida. 1 Test Materials 1.1 fruiting body collected in Kunming whistle of fresh mature fruiting body (gray or brown, broom-like small branches obvious, the top branchlets more than 5cm) and young fruiting bodies (bright yellow, no coral branches , Diameter 0.5 ~ 2cm). 1.2 Medium Inclined medium 20g potato, glucose 20g, 0.5g magnesium sulfate, potassium dihydrogen phosphate 0.25g, thiamine 30μg, agar 20g, water 1000mL, sterilized at 121 ℃ for 30 minutes made of bevel. A medium: the above sterilization medium melting, slightly cooled in a sterile room with streptomycin 50μg / mL, shake, into sterile petri dishes, each dish 1O ~ 15mL, thickness